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Introduction of Phage Display Technology Phage display is the technology that inserts the DNA sequence of foreign protein or peptide into the appropriate position of phage coat protein structural gene, so that the exogenous gene can be expressed along with the expression of phage coat protein itself. Meanwhile, the foreign protein also can be shown in the surface of phage coat protein following the reassembly of phage. In short, a phage library expressing a wide diversity of peptides or proteins is used to select those binding the desired targets.

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Process Service Items Service Cycle 1 Separation of lymphocytes Obtain lymphocytes from peripheral blood, spleen or bone marrow. 1 week 2 RNA isolation and cDNA synthesis RNA extraction, then reverse transcription into cDNA. 3 Construction of recombinant plasmid With cDNA as the template, the antibody gene will be extended with degenerate primer, then inserted into the phage vector.

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